Growth Dynamics of E. coli in Varying Concentrations of Nutrient

Abstract The purpose in this experiment of growth kinetics of E. coli in varying media was to determine which media produces the maximum digit of kioskular phones per building block of measurement time. first gear a snub back was established for E. coli in a 1.0x food broth. This was use to comp be the growth in the experimental media of 0.5x and 2.0x, food broths; alimental broths with an additional 5.0mM of glucose and another with 5.0mM lactose; nutrient broths of varying pH levels: 6.0, 7.0, and 8.0; and in conclusion a nutrient broth in the presence of the drug/antibiotic chloramphenicol. A variety of OD readings were taken and calculations made to determine the deed of cells present after(prenominal) a given time. Then two graphs were plotted, Number of cells per unit volume versus Time in minutes and record of the number of cells per unit volume versus Time growth curve. The final cell concentration for the tick was 619,500 cells/mL. Four media, afte r calculations, produced fewer cells than that of the control, these were: chloramphenicol producing 89,301 cells/ml; glucose producing 411,951 cells/mL; lactose producing 477,441 cells/mL and eventually pH 6.0 producing 579,557cells/mL. The remaining four media, after calculations, produced cell counts greater than the control: 2X with 1,087,009 cells/mL; 0.5X with 2,205,026 cells/mL; pH 8 with 3,583,750 cells/mL and finally pH 7.0 with 8,090,325 cells/mL.

From these results the conclusion can be made that the milieu is a controlling factor in the growth kinetics of E. coli. This was found by dint of the regulation of pH and nutrient concentrations. In the ! presence of the drug/antibiotic, chloramphenicol, cell growth was minimal. Introduction E. coli grows and divides through vegetal reproduction. Growth will continue until all nutrients are expend and the wastes rise to a toxic level. This is demonstrated by the Log of the number of cells per... If you want to get a generous essay, rig it on our website: OrderCustomPaper.com

If you want to get a full essay, visit our page: write my paper